science hard true/false

True or False: CRISPR-Cas9 can be used for both gene editing and gene regulation without cutting DNA.

  1. True
  2. False

Answer: True

Dead Cas9 (dCas9) fused with activators/repressors can regulate gene expression without DNA cleavage (CRISPRa/i). Standard Cas9 cuts DNA for editing. This versatility expands therapeutic applications. [[DBT Reports, NCERT Biology Class 12]]

Topic Biotechnology - Gene Therapy
Exam Relevance UPSC CSE, SSC Scientific Assistant, CSIR NET

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